TRapamycin reduces testicular ischemia-reperfusion injury by enhancing autophagy-Reference-Cited by-同舟云学术

TRapamycin reduces testicular ischemia-reperfusion injury by enhancing autophagy

Published:2024-05-17 Issue: Volume: Page:
ISSN:2788-6859
Container-title:Asia-Pacific Journal of Pharmacotherapy & Toxicology
language:en
Short-container-title:Asia-Pac J Pharmacother Toxicol

Author:

Hu Zhi¹,Cheng Qiong²,Xu Lv¹,Chen Yiyan¹,Ning Jinzuo³,Cheng Fan³,Zhang Wei¹

Affiliation:

1. Department of Urology, Tongren Hospital of Wuhan University, Wuhan Third Hospital, Wuhan, Hubei 430060, China.

2. Department of Medical Ultrasonics, Hubei Maternal and Child Health Hospital, Wuhan, Hubei 430060, China.

3. Department of Urology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, China.

Abstract

Objectives To confirm the effects of autophagy on testicular ischemia-reperfusion (I/R) injury. Methods Forty rats were divided into sham group, I/R group, I/R+Rap (rapamycin, autophagy activator) group and I/R+ 3-MA (3-methyl adenine, autophagy inhibitor) group. Before inducing ischemia, rapamycin and 3-MA were intraperitoneally injected into I/R+Rap and I/R+ 3-ma groups, respectively. Subsequently, we then assessed testicular tissue damage. Immunohistochemistry was used to detect Beclin-1 and Caspase-3, while Western blot and qRT-PCR detected LC-II, Beclin-1 and p62. TUNEL and transmission electron microscopy were used to observe apoptosis and autophagosome. Results I/R activated autophagy in rat testicles. Rapamycin significantly improved testicular function after I/R by enhancing autophagy, reducing spermatogenic cell apoptosis, and decreasing testicular tissue damage scores. Conclusions Enhancing autophagy has a protective effect in ischemic-reperfusion injury by reducing apoptosis of rat testicular sperm cells.

Publisher

Asian Medical Press Limited

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