Toxocara Species Infection in Pet Dogs and Cats Ensured by Loop-Mediated Isothermal Amplification Assay

Abstract

Background: Toxocariasis is a parasitic disease with global distribution. Many paratenic or transport hosts have been identified as the sources of infection, including vertebrates such as birds and humans. The definitive hosts of Toxocara are dogs and cats whose parasites live in their guts. Hence, the diagnosis of the disease in these animals is important. Objective: The present study was performed using the loop-mediated isothermal amplification (LAMP) method to diagnose toxocariasis. Materials and Methods: In the present study, 60 samples of pet feces were collected and subsequently evaluated by direct smear, formalin-ether, and LAMP. Results: Toxocara infection in dogs by microscopic and formal ether methods and LAMP method was 10% and 13.3%, respectively, while feline infection with this parasite was not observed either microscopically or molecularly. Conclusion: The present findings revealed that pet dogs can be considered as a source of infection for humans. Therefore, it is recommended that animal owners receive the necessary training to prevent the risks of transmitting zoonotic diseases.