Antibacterial, bacteriolytic, antibiofilm, and synergistic effects of Curcuma species ethanol extracts with antibiotic against multidrug resistant Pseudomonas aeruginosa

Author:

Yuandani Yuandani1ORCID,Septama Abdi Wira2ORCID,Utami Dinda Sari3ORCID,Nugraha Sony Eka4ORCID,Sufitni Sufitni5ORCID,Khairunnisa Nur Aini6ORCID,Nasution Halimah Raina6ORCID,Rizka Ramaza7ORCID

Affiliation:

1. Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia

2. Research and Innovation Agency (BRIN), Kawasan PUSPIPTEK Serpong, Tangerang 8 Selatan, Banten 15314, Indonesia

3. Department of Pharmacy, Universitas Muslim Nusantara Al Washliyah, North Sumatera, Indonesia

4. Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia

5. Department of Anatomy, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia

6. Graduate School of Master in Pharmaceutical Science, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia

7. Department of Hospital Pharmacy, dr. Zainoel Abidin General Hospital, Banda Aceh, Indonesia

Abstract

Introduction: Curcuma species have shown antibacterial activity against Pseudomonas aeruginosa. The current study was conducted to analyze the antibacterial activity of ethanol extracts of Curcuma species rhizomes, including Curcuma domestica, C. xanthorrhiza, C. mangga, C. zedoaria, and C. aeruginosa against multidrug-resistant Pseudomonas aeruginosa (MDR P. aeruginosa). Furthermore, the mechanism action of Curcuma species in combination with antibiotic against MDR P. aeruginosa and its chemical component were also investigated. Methods: Determination of minimum inhibitory concentration (MIC) was carried out by the microdilution method. The synergistic effects of the extract and tetracycline were determined by the checkerboard method. The effect of the combination of Curcuma species and tetracycline to prevent bacterial resistance was investigated using inhibition of biofilm formation, permeability of bacterial cell membrane, and EtBr accumulation methods. Gas chromatography-mass spectrometry (GC-MS) analysis was also performed. Results: MIC of C. domestica, C. xanthorrhiza, and C. mangga against MDR P. aeruginosa were 125, 250, and 125 µg/mL, respectively. C. xanthorrhiza ethanol extract (7.8 µg/mL) in combination with tetracycline (1.9 µg/mL) revealed a synergistic activity with Fractional Inhibitory Concentration Index (FICI) value of 0.06. The combination of C. xanthorrhiza ethanol extract and tetracycline showed inhibitory effects on biofilm formation and efflux pump of MDR P. aeruginosa. This combination also had bacteriolytic activity. GC-MS analysis led to the identification of ar-turmerone (11.63%) and xanthorrhizol (11.36%) as the major compounds. Conclusion: Combination of C. xanthorrhiza ethanol extract and tetracycline might be developed as an alternative treatment against MDR P. aeruginosa.

Publisher

Maad Rayan Publishing Company

Reference34 articles.

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3