In-depth hepatoprotective mechanistic study of Echinacea purpurea flowers: In vitro and in vivo studies-Reference-Cited by-同舟云学术

In-depth hepatoprotective mechanistic study of Echinacea purpurea flowers: In vitro and in vivo studies

Published:2021-11-29 Issue:1 Volume:11 Page:99-106
ISSN:2345-5004
Container-title:Journal of Herbmed Pharmacology
language:en
Short-container-title:J Herbmed Pharmacol

Author:

Shalaby Mostafa Abass¹^ORCID,Elbanna Hossny Awad¹^ORCID,Mohamed Salma Mostafa¹^ORCID,Nabil Ghazal A¹^ORCID,Elbanna Ahmed Hossny²^ORCID

Affiliation:

1. Pharmacology Department, Faculty of Veterinary Medicine, Cairo University, Egypt

2. Pharmacology Department, Michael Sayegh Faculty of Pharmacy, Aqaba University of Technology, Jordan

Abstract

Introduction: Echinacea purpurea is a flowering plant commonly used as an herbal medicine despite insufficient scientific bases to validate its usage. The present study aimed to examine in vitro and in vivo hepatoprotective effects of aqueous and alcoholic extracts of E. purpurea flowers. Methods: In vitro protection against hepato-cytotoxicity was carried out on human HepG-2 cells using colorimetric tetrazolium (MTT) assay, while the in vivo hepatoprotective activity was studied against carbon-tetrachloride (CCl4) induced acute hepatotoxicity in rats. Results: The results revealed that the extracts of E. purpurea induced discernable in vitro protection on HepG-2 cells and in vivo against CCl4 induced hepatotoxicity. Both extracts were significantly able to restore the serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total bilirubin, total protein, and albumin to normal levels compared to the CCl4 intoxicated group. In addition, the extracts markedly mitigated the oxidative stress by decreasing Malondialdehyde (MDA) and increasing superoxide dismutase (SOD) and glutathione (GSH) markers compared to the CCl4 intoxicated group. It was also associated with the down-regulation of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels in liver tissues. Histopathological examination revealed a decrease in hepatocytes’ degenerative changes and noticeable improvement of the liver damage by extracts of E. purpurea. Conclusion: These findings have proven that aqueous and alcoholic extracts of E. purpurea flowers have a significant hepatoprotective effect, probably owing to antioxidant, anti-inflammatory activities, and regulating apoptotic-related genes. This confirms the ethnomedicinal uses of E. purpurea in patients suffering from liver diseases.

Publisher

Maad Rayan Publishing Company

Subject

Drug Discovery

Link

http://herbmedpharmacol.com/PDF/jhp-11-99.pdf

Reference44 articles.

1. Dienstag JL, Isselbacher KJ. Toxic and drug-induced hepatitis. In: Kasper DL, Braunwald E, Fauci A, Hauser S, Longo D, Jameson JL, eds. Harrison’s Principles of Internal Medicine. 19th ed. New York: McGraw-Hill Professional; 2015.

2. Mesalazine, an osteopontin inhibitor: The potential prophylactic and remedial roles in induced liver fibrosis in rats

3. Rosmarinic acid-a new hope for liver diseases like cirrhosis, hepatocellular carcinoma-needs translation to humans;Kaur KK;EC Endocrinol Metab Res,2019

4. Molecular mechanisms underlying hepatoprotective effect of artichoke extract: modulates tnf-induced activation of nuclear transcription factor (NF-KAPPA ẞ) and oxidative burst inhibition;Afifi N;World J Pharm Phram Sci,2015