A Simple Colorimetric Method for Determination of Ethanol in Exhaled Breath Condensate-Reference-Cited by-同舟云学术

A Simple Colorimetric Method for Determination of Ethanol in Exhaled Breath Condensate

Published:2020-05-27 Issue:2 Volume:27 Page:297-301
ISSN:1735-403X
Container-title:Pharmaceutical Sciences
language:en
Short-container-title:Pharm Sci

Author:

Pourkarim Fariba¹^ORCID,Rahimpour Elaheh²³^ORCID,Khoubnasabjafari Maryam⁴^ORCID,Jouyban-Gharamaleki Vahid⁵^ORCID,Farhang Sara⁶^ORCID,Jouyban Abolghasem²⁷^ORCID

Affiliation:

1. Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

2. Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.

3. Food and Drug Safety Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

4. Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

5. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

6. Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.

7. Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran.

Abstract

Background: Ethanol is considered as a toxic compound when used in excess amounts. The toxic concentration for ethanol was reported to be 1000 – 2000 μg.mL-1 in plasma and serum samples. The aim of the current study was to develop a rapid and catalyst free colorimetric method for determination of ethanol in exhaled breath condensate (EBC) sample. Methods: A redox reaction with dichromate-based colorimetric method was used for determination of ethanol in EBC. Results: The proposed method shows a good sensitivity and selectivity for ethanol in compared with other compounds and biomarkers existing in EBC. The color change can be easily observed by the naked eye in the presence of ethanol in the range of 300 - 8000 μg.mL-1. The quantitative detection of ethanol was fully validated and used for determination of ethanol in EBC of alcohol administrated individuals. Conclusion: This catalyst free colorimetric method has great potential for ethanol determination owing to many desirable properties such as high reliability, high sensitivity, and fast response time.

Publisher

Maad Rayan Publishing Company

Subject

General Pharmacology, Toxicology and Pharmaceutics,Pharmaceutical Science

Link

https://ps.tbzmed.ac.ir/PDF/ps-27-297.pdf

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