Abstract
Aim. To estimate the effect of ionol (2,6-di-teRt-butyl-4-methylphenol) on the dynamics of lens opacities and its free-radical status in experimental cataract.
Methods. Cataract was induced by a single injection of diquat dibromide solution into the vitreous body of 80 rabbits (160 eyes) at a dose of 600 nmol, 5 rabbits were included into the control intact group. Animals with induced cataract were divided into 4 groups each including 20 animals (40 eyes) who were treated with TID instillation in conjunctival cavity of: olive oil (control), «Oftan Catachrom», solution of α-tocopherol acetate, 2.2% ionol oil solution. Treatment was started on day 7. The duration of the experiment was 56 days. Dynamics of lens opacities was assessed by the following criteria: progress, stabilization and regression. In the lens homogenate the concentration of malonic dialdehyde, level of non-protein thiol (SH) groups, activity of glutathione peroxidase and glutathione transferase were determined. The results were analyzed by ANOVA, paired comparison was performed by the Newman-Keysl criterion.
Results. Application of ionol reduced malonic dialdehyde concentration in the lens compared to the series without treatment on days 14, 28, 42 and 56 (by 74.3, 90.0, 89.3, and 80.2%, p