Alcohol and aldehyde dehydrogenase activity in the stomach and small intestine of rats poisoned with methanol-Reference-Cited by-同舟云学术

Alcohol and aldehyde dehydrogenase activity in the stomach and small intestine of rats poisoned with methanol

Published:2001-05 Issue:5 Volume:20 Page:255-258
ISSN:0960-3271
Container-title:Human & Experimental Toxicology
language:en
Short-container-title:Hum Exp Toxicol

Author:

Chrostek L,Szczepura D¹,Szmitkowski M²,Jelski W³,Wierzchowski J¹

Affiliation:

1. Department of Biochemical Diagnostics, Medical Academy, Bialystok, Poland

2. Department of Biochemical Diagnostics, Medical Academy, Bialystok, Poland; Department of Biochemical Diagnostics, Medical Academy, Waszyngtona 15A, Bialystok, Poland

3. Department of Organic Chemistry, Pedagogical University, Siedlce, Poland

Abstract

The activities of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) were measured with fluorogenic naphthaldehydes in the stomach and small intestine homogenates of rats dosed with 6 g methanol/kg bw after 6, 12, 24 h and 2, 5, 7 days. After intoxication with a sublethal dose, the ADH activity measured with these naphthaldehydes andALDH activities in the stomach and small intestine were significantly decreased. This inhibition is stronger in the stomach and probably depends on cell damage and protein denaturation. We conclude that the activity measured with 6-methoxy-2-naphthaldehyde (MONAL-62) may be due to the activity of rat ADH-1 isoenzyme, and the activity detected with 4-methoxy-1-naphthaldehyde (MONAL-41) to the activity of rat ADH-2 isoenzyme.

Publisher

SAGE Publications

Subject

Health, Toxicology and Mutagenesis,Toxicology,General Medicine

Link

http://journals.sagepub.com/doi/pdf/10.1191/096032701678227703

Reference16 articles.

1. The toxicity of methanol

2. Different forms of rat liver aldehyde dehydrogenase and their subcellular distribution

3. Characterization of three isoenzymes of rat alcohol dehydrogenase. Tissue distribution and physical and enzymatic properties

4. The intramucosal distribution of gastric alcohol dehydrogenase and aldehyde dehydrogenase activity in rats

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