Evaluation on the efficacy of neural stem cells supernatant combined with AKT inhibitor in intracerebral hemorrhage model in vitro

Abstract

Objective: Intracerebral hemorrhage (ICH), charactered by bleeding and subsequent cortical neuronal injury, is a kind of cerebrovascular disease with high disability and mortality. Although neural stem cells (NSC) transplantation emerges as a potential therapy for ICH by protecting injured cortical neurons, its efficacy remains limited. It is acknowledged that AKT (Protein Kinase B, PKB) inhibitors, as classical cytostatic agents, play a role in a variety of biological processes, including cell growth, proliferation, differentiation, and apoptosis. Therefore, this study aims to evaluate the combined effects of NSC supernatant and AKT inhibitors on cortical neurons in ICH. Methods: Primary cortical neurons were cultured and treated with hemin chloride (hemin) to induce ICH in vitro model. The combined strategy of both NSC supernatant and AKT inhibitor was applied. Cell viability was assessed using CCK-8 assays, and apoptosis was evaluated through Hoechst 33342/PI co-staining. Statistical analysis was conducted using SPSS 17.0 software. Results: The combination of NSC supernatant and AKT inhibitors significantly enhanced cell viability and reduced apoptosis in hemin-induced cortical neuronal injury compared to individual treatments. The synergistic effect was demonstrated by increased cell counts and viability in the NSC supernatant + AKT inhibitor group. Conclusion: NSC supernatant combined with AKT inhibitors synergistically enhances therapeutic and anti-apoptotic effects in cortical neuronal injury induced by hemin, showing a potential as a novel therapeutic strategy for neuronal injury in ICH models.