Adiponectin and the expression of BAX and caspase 3 in high-fructose - induced testicular injury in albino mice

Abstract

Introduction and Aim: The increase in the prevalence of obesity and metabolic syndrome in recent decades has been correlated with high consumption of high-fructose and high-fat diets and has been associated with increased rates of male infertility. The aim of this study was to investigate how high fructose diet exerts its effect upon testicular morphology in addition to examine the potential effects of adiponectin treatment in restoring the architecture of seminiferous tubules through the expression of immunohistochemical markers BAX and caspase-3. Materials and Methods: Twenty-five adult albino mice were divided into three groups: In Group 1, mice fed with diet contained high concentration of fructose followed by adiponectin injection, Group 2, the mice fed with high concentration of fructose diet and received a saline placebo injection, and Group 3 (control) was nourished a regular food for 8 weeks. The parameters studied included changes in animal body weight, testicular spermatogenesis index, spermatogonia count, apoptotic index, exfoliative epithelium percentage and immunohistochemical scores for testicular BAX and caspase-3 expression. Results: Animals on high fructose diet showed increase in body weight which was markedly reduced by adiponectin treatment. High fructose diet also resulted in reduced spermatogenesis index and spermatogonia count with increased apoptotic and epithelial exfoliation indices. High fructose diet was also associated with high-fructose induced obesity and significantly associated with increased BAX and caspase-3 expression alleviated by adiponectin treatment. Conclusion: High-fructose intake induces obesity and obesity-related reduction in male fertility by reducing spermatogenesis and enhancing testicular cell apoptosis via different pathophysiological mechanisms. Such effects and mechanism can be reversed and corrected with adiponectin treatment.