Generation of bicistronic reporter knockin mice for visualizing germ layers

Author:

Suzuki Hayate12,Dinh Tra Thi Huong1,Daitoku Yoko1,Tanimoto Yoko1,Kato Kanako1,Azami Takuya3,Ema Masatsugu3,Murata Kazuya1,Mizuno Seiya1,Sugiyama Fumihiro1

Affiliation:

1. Laboratory Animal Resource Center, Trans-Border Medical Research Center, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan

2. Doctor’s Program in Biomedical Sciences, Graduate School of Comprehensive Human Science, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan

3. Department of Stem Cells and Human Disease Models, Research Center for Animal Life Science, Shiga University of Medical Science, Seta, Tsukinowa-cho, Otsu, Shiga 520-2192, Japan

Publisher

Japanese Association for Laboratory Animal Science

Subject

General Veterinary,General Biochemistry, Genetics and Molecular Biology,Animal Science and Zoology,General Medicine

Reference28 articles.

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2. 2. Anand T., Talluri T.R., Kumar D., Garrels W., Mukherjee A., Debowski K., Behr R. and Kues W.A. 2016. Differentiation of Induced Pluripotent Stem Cells to Lentoid Bodies Expressing a Lens Cell-Specific Fluorescent Reporter. PLoS One 11: e0157570.

3. 3. Ang S.L., Conlon R.A., Jin O. and Rossant J. 1994. Positive and negative signals from mesoderm regulate the expression of mouse Otx2 in ectoderm explants. Development 120: 2979–2989.

4. 4. Burtscher I., Barkey W., Schwarzfischer M., Theis F.J. and Lickert H. 2012. The Sox17-mCherry fusion mouse line allows visualization of endoderm and vascular endothelial development. Genesis 50: 496–505.

5. 5. Cong L., Ran F.A., Cox D., Lin S., Barretto R., Habib N., Hsu P.D., Wu X., Jiang W., Marraffini L.A. and Zhang F. 2013. Multiplex genome engineering using CRISPR/Cas systems. Science 339: 819–823.

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