Utilization of the CRISPR/Cas9 system for the efficient production of mutant mice using crRNA/tracrRNA with Cas9 nickase and FokI-dCas9
Author:
Affiliation:
1. Department of Systems BioMedicine, National Research Institute for Child Health and Development, Tokyo 157-8535, Japan
2. Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan
Publisher
Japanese Association for Laboratory Animal Science
Subject
General Veterinary,General Biochemistry, Genetics and Molecular Biology,Animal Science and Zoology,General Medicine
Link
https://www.jstage.jst.go.jp/article/expanim/65/3/65_15-0116/_pdf
Reference24 articles.
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2. 2. Bassett A.R., Tibbit C., Ponting C.P., and Liu J.L. 2013. Highly efficient targeted mutagenesis of Drosophila with the CRISPR/Cas9 system. Cell Reports 4: 220–228.
3. 3. Cheng A.W., Wang H., Yang H., Shi L., Katz Y., Theunissen T.W., Rangarajan S., Shivalila C.S., Dadon D.B., and Jaenisch R. 2013. Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cell Res. 23: 1163–1171.
4. 4. Cong L., Ran F.A., Cox D., Lin S., Barretto R., Habib N., Hsu P.D., Wu X., Jiang W., Marraffini L.A., and Zhang F. 2013. Multiplex genome engineering using CRISPR/Cas systems. Science 339: 819–823.
5. 5. Deltcheva E., Chylinski K., Sharma C.M., Gonzales K., Chao Y., Pirzada Z.A., Eckert M.R., Vogel J., and Charpentier E. 2011. CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III. Nature 471: 602–607.
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