Analysis of the transgene insertion pattern in a transgenic mouse strain using long-read sequencing
Author:
Affiliation:
1. Laboratory of Animal Models for Human Diseases, National Institutes of Biomedical Innovation, Health and Nutrition,7-6-8 Saito-Asagi, Ibaraki, Ibaraki, Osaka 568-0085, Japan
Publisher
Japanese Association for Laboratory Animal Science
Subject
General Veterinary,General Biochemistry, Genetics and Molecular Biology,Animal Science and Zoology,General Medicine
Link
https://www.jstage.jst.go.jp/article/expanim/69/3/69_19-0118/_pdf
Reference40 articles.
1. 1. Behringer R., Gertsenstein M., Nagy K.V. and Nagy A. 2014. Genotyping. pp. 551–570. In: Manipulating the mouse embryo: a laboratory manual, 4th ed., Cold Spring Harbor Press, New York.
2. 2. Bishop J.O. 1996. Chromosomal insertion of foreign DNA. Reprod. Nutr. Dev. 36: 607–618.
3. 3. Bishop J.O. and Smith P. 1989. Mechanism of chromosomal integration of microinjected DNA. Mol. Biol. Med. 6: 283–298.
4. 4. Cain-Hom C., Splinter E., van Min M., Simonis M., van de Heijning M., Martinez M., Asghari V., Cox J.C. and Warming S. 2017. Efficient mapping of transgene integration sites and local structural changes in Cre transgenic mice using targeted locus amplification. Nucleic Acids Res. 45: e62.
5. 5. Callis T.E., Pandya K., Seok H.Y., Tang R.H., Tatsuguchi M., Huang Z.P., Chen J.F., Deng Z., Gunn B., Shumate J., Willis M.S., Selzman C.H. and Wang D.Z. 2009. MicroRNA-208a is a regulator of cardiac hypertrophy and conduction in mice. J. Clin. Invest. 119: 2772–2786.
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