Affiliation:
1. Rostov research institute of oncology
Abstract
Improved knowledge of molecular mechanisms of the damaging effect of thermal ischemia and reperfusion to renal tissue of patients with renal cancer has significant prospects for new therapeutic approaches aimed at enhancing quality of care. THE AIM: to study changes in the expression of apoptosis-regulating genes MDM 2, BAX, CASP7, CASP3, CASP8, CASP9, BCL2, CASP8 / FADD, p53, APAF1, AIFM1, ICAD and XIAP in renal tissue of patients with renal cell carcinoma subjected to the action of ischemia and reperfusion. PATIENTS AND METHODS. We used for the study tissue biopsies of 12 patients with histologically confirmed diagnosis of renal cancer. Needle biopsy was performed before stop the blood supply, for 10 minutes of ischemia and 20 minutes after reperfusion in the kidney. The relative expression of genetic loci was determined by real-time PCR. RESULTS. It was found: 1) absence from the 10th minute of ischemia significant differences transcriptome profile of the majority of investigated genes from similar parameters prior to ischemia, with the exception of reducing expression of genes CASP7 and ICAD; 2) a significant increase in expression of pro-apoptotic genes (BAX, CASP3/7, APAF1 and p53), and anti-apoptotic genes (XIAP, MDM2 and BCL2) 20 minutes after reperfusion of the kidney tissue; 3) a changes in the balance of expression of pairs of proand anti-apoptotic genes p53 / MDM2 and Bax / BCL2 in the 10th minute of ischemia and 20 minutes after reperfusion. CONCLUSION. These data characterize transcriptomic state of renal tissue in the early period after ischemia and restore the blood flow in it as the initiation point shift the balance of proand anti-apoptotic genes.
Publisher
Non-profit organization Nephrology
Reference22 articles.
1. Matveev VB, Perlin DV, Figurin KM, Volkova MI. Organosokhranyayushchee lechenie raka pochki. Prakt onkol 2005; (3):162-166 [Matveev VB, Perlin DV, Figurin KM, Volkova MI. Organosohranyayuschee lechenie raka pochki. Prakticheskaya onkologiya 2005; (3):162-166]
2. Smirnov AV, Kayukov IG, Dobronravov VA, Rumyantsev ASh. Ostroe povrezhdenie pochek: kontseptual'nye problemy. Nefrologiya 2014;18(2):8-24 [Smirnov AV, Kaiukov IG, Dobronravov VA, Rumyantsev ASh. Ostroe povrezhdenie pochek: kontceptualnye problemy. Nefrologiia 2014;18(2):8-24]
3. Liano F, Pascual J. Madrid acute renal failure study group. Epidemiology of acute renal failure: A prospective, multicenter, community-based study. Kidney Int 1996; (50): 811–818
4. Sheridan AM, Bonventre JV: Cell biology and molecular mechanisms of injury in ischemic acute renal failure. Curr Opin Nephrol Hypertens 2000; (9): 327–434
5. Zhang Y, Fu Z, Zhong Z et al. Hypothermic Machine Perfusion Decreases Renal Cell Apoptosis During Ischemia/Reperfusion Injury via the Ezrin/AKT Pathway. Artificial Organs 2015 doi: 10.1111/aor.12534
Cited by
4 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献