Laboratory rats mesenteric vessel and intestine microcirculation intravital microscopy using original local abdominal decompression unit

Abstract

Introduction. The local decompression therapy (including local abdominal decompression) is known to contribute to the intensity of capillary blood flow and improve the functional state of the microvasculature. We have proposed a development could be used for intravital investigation of changes in microcirculation directly under local negative pressure conditions.Purpose. Investigation of microcirculatory changes under the conditions of local negative pressure in the rat intestine and mesentery combined with pharmacological effects.Materials and methods. Unit for vital microscopy under local negative pressure was used for this work. The specific of the unit is the ability of continuous record the field of view of the microscope before, during and after the decompression session. In the experiment there were used Wistar rat males weighing 300–450 g, distributed into 5 groups of 5 animals. The animals were divided into groups depending on the pharmacological impact: atropine, pilocarpinum, drotaverine and chloropyramine and the control group.Results. It has been shown that dynamics of vascular constriction and dilatation after decompression session varies in response of certain agents suggested the involvement of certain receptor fields. The changing vessel number within the video recording frames by groups of animals was analyzed at different stages of exposure: before decompression, during decompression, within 1 minute after exposure, as well as in the interval from 1 to 5 minutes and from 5 to 19 minutes after exposure. In all groups vasoconstriction preceded decompression (i. e., reduced number of vessels in the frame), but in all of them vasodilation (increased number of vessels in the frame) occurred in the first minute after decompression. Interestingly, in the «drotaverine» group, the dynamics of changes was most pronounced: unlike other groups, vasoconstriction (0,24±0,177 %) continued during decompression, and expansion right after exposure was also highly expressed (1.034±0.997 %).Conclusion. hyperemic changes and the severity of prolongation of these changes was shown to be different depending on the type of pharmacological effect and could be recorded by the experimental unit developed by the authors.