Affiliation:
1. Ryazan State Medical University named after Academician I. P. Pavlov
Abstract
Introduction. One of the promising directions of studying liver microcirculation is searching for age-related features of its hemodynamics and early markers of pathological changes. Objective – to study the parameters of microcirculation in the liver of animals of different sex and age during a load test. Materials and methods. The study was conducted on 60 mongrel rats of both sexes weighing 120–270 g, divided into three age groups. The first group included 20 rats aged 1 month, the second – 20 rats aged 6–12 months and the third – 20 rats aged more than 18–24 months. The study of microcirculation was performed using a laser Doppler flowmeter LAKK-02 (Russia) under zoletil-xylazine anesthesia. Then, the rats were injected per os with mixture for enteral nutrition Nutridrink (Nutricia) in a ratio of 5 mg/kg of body weight. After 15 minutes the microcirculation parameters were determined again. Digital data were processed by methods of variation statistics. Results. In the liver of rats, the basic parameters of microcirculation were slightly higher in females compared to males: by 8.22 % (R=0.31) in the I, by 8.66 % (R= 0.05) in the II and by 1.19 % (R=0.48) in the III age group. With an increase in the age of the animals, the microcirculation indicators decreased. After the load test, an increase in microcirculation indexes was noted in the liver of rats in all age groups: by 45.13 % (R=0.82) in the I, by 27.99 % (R=0.64) in the II and by 15.88 % (R=0.91) in the III age group. Conclusion. There was no statistically significant dependence of the parameters of microcirculation in the liver of rats on the sex of the animals. With an increase in the rat’s age, the microcirculation rates decreased. After a food load test in the liver of rats, a statistically significant increase of the parameters of microcirculation was noted in all age groups. With an increase in the age of animals, the increase in microcirculation rates after a food load test significantly decreased.
Publisher
FSBEI HE I.P. Pavlov SPbSMU MOH Russia
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