A new protocol for isoheart rat kydney blood perfusion

Abstract

Introduction and the purpose of the work. The perfusion of isolated laboratory animal organs is important for estimation of the effectiveness of methods elaborated for donor organ preservation. Materials and methods. Hemoperfusion of isolated rat kidney was performed after long-term hypothermal ischemia in order to reproduce reperfusion for the subsequent estimation of morphological changes in the cloth of nephritic parenchyma. Results. This method proved to be effective and technically simpler than different modifications of the classical procedure of kidney transplantation in small laboratory animals. Conclusions. The proposed procedure is an alternative to the classical kidney transplantation if reperfusion is required in an acute experiment for several hours.