The changes in the oxygen transport capability of erythrocyte hemoglobin in patients with ischemic stroke

Abstract

The statistics of acute cerebrovascular accident (ACC) has been disappointing for decades. It is especially interesting to study the oxygen transport properties of blood under stroke as a probable mechanism for progression of acute hypoxia of the nervous tissue. The aim of this research was to study changes in the structure and oxygen transport capacity of hemoglobin in patients with varying degrees of ischemic stroke severity using RAMAN spectroscopy. Materials and methods. There was a clinical cross-sectional observational study of patients (n=60) with stroke of ischemic origin. We did standard diagnostic procedures, RAMAN spectroscopy of blood smears (inVia™ Basis spectrometer (Renishaw, UK, laser wavelength 532 nm, power 1.5 mW), statistical analysis of the data. Results. The conformational structure of hemoporphyrin in erythrocytes of patients with stroke has significant differences from the results of relatively healthy donors, corresponding to the severity of the pathology. The content of oxyhemoglobin decreases by 7.1–16.1 % (p<0.05) relative to the control. The ability of hemoglobin to bind and release ligands increases by an average of 34.7–47.2 % (p<0.05) and 9.9–23.9 % (p<0.05), respectively. Ligand affinity increases by 30.1 %, 29.0 % and 47.3 % (p<0.05) under mild, moderate and severe forms of the disease. Conclusion. In the erythrocytes of patients with stroke of ischemic origin, the conformation of hemoglobin changes, its affinity for oxygen increases, and its oxygen transport function is modified. The revealed changes correlate with the severity of the stroke.