Extraction and Characterization of β-Glucan from Kombucha Bacterial Cellulose and Concentrated Kombucha: Evaluating their Bile Acid Binding Capacity
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Published:2023-05-31
Issue:3
Volume:50
Page:1-11
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ISSN:0125-2526
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Container-title:Chiang Mai Journal of Science
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language:
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Short-container-title:CMJS
Author:
Charoenrak Sonthirat, ,Wakayama Mamoru,Charumanee Suporn,Sirisa-ard Panee,Bovonsombut Sittisin,Kiatkarun Suwalee,Chitov Thararat,Bovonsombut Sakunnee, , , , , , , , ,
Abstract
β-glucan is a water-soluble polysaccharide present in natural products and has many health benefi ts such as its role as a dietary fi ber. This study aimed to extract and characterize β-glucan in kombucha bacterial cellulose (KBC) powder and concentrated kombucha. The extraction method used was ultrasound sonication combined with enzymatic treatment. The results showed that the content of β-glucan was 19.70% (w/w) in KBC extract and 8.34% (w/v) in concentrated kombucha extract, determined using β-glucan assay kit (Mixed linkage). Fourier-transform infrared (FTIR) spectroscopy analysis confi rmed the presence of β-glucan with β-(1→3) and β-(1→4) glucan linkages, as well as the presence of proteins, mannans, and other components in both KBC β-glucan and concentrated kombucha β-glucan extracts. Regarding bile acid binding potential, concentrated kombucha β-glucan exhibited a high bile acid binding capacity for up to 24 hours, while KBC β-glucan only retained this capacity for up to 4 hours. Altogether, these fi ndings highlight the presence and properties of β-glucan in kombucha, suggesting its potential as a functional ingredient with health benefi ts.
Publisher
Chiang Mai University
Subject
General Physics and Astronomy,General Biochemistry, Genetics and Molecular Biology,General Materials Science,General Mathematics,General Chemistry