Vitiligo is a multifactorial disorder disease characterized by loss of functional melanocytes, genetic damage, excess inflammation, autoimmune dysfunction, and extreme oxidative stress in lesions. Unfortunately, there is not enough response to obtainable therapies nowadays. The present study was designed using a plasma jet powered by a Tesla coil, which can be used for generating cold atmospheric pressure plasma jet (CAPPJ) from dielectric barrier discharge. The subject samples were categorized into two groups, first one was normal blood samples (control) and the second was vitiligo patient's blood samples. Present study parameters were as a following: cytokinesis blocked micronucleus test cytome assay, protein expression of both P53 and Bcl2 genes, interleukins (IL-1β, IL-6, and IL-10) and tumor necrosis factor-alpha. The study plans to investigate the effect of CAPPJ on whole blood cultures of vitiligo patients compared with matched control blood cultures. CAPPJ affects white blood cells (lymphocytes) and reduces its production of inflammatory cytokines, which cascade the autoimmune response. In addition, CAPPJ successfully reduces Mni frequencies, enhancing apoptosis and normalizing P53 and Bcl2 genes expression in vitiligo samples. The current study showed that CAPPJ exposure can achieve enhanced treatment of some types of autoimmune diseases such as vitiligo through molecular mechanisms of action, cell cycle regulation, activation of cell signaling pathways, and/or pleiotropic antioxidant and anti-inflammatory properties.