Genetic Relationship Between Chronic Otitis Media Infection And TLR4 Gene In AL-Qadisyah province, Iraq .

Abstract

Background :otitis media is considered the more diseases common spread  related  hearing loss ,This study is designed to focus on TLR4 expression  that stimulating result presence LPS in otitis media Objective :detect the genotype more infection in otitis media on blood patients sample   expression  in PCR Technical. Methods :The current study blood specimens we get of 50 patient and  chronic Otitis media and 50 blood samples from healthy people to Extracting and dissection of the DNA blood gram negative bacteria and  evaluate the mechanical and work of LPS and its effect on Toll like receptor4. Results :The results show most patients with P. arogenosia isolates have GG genotype 9 in comparison with other genotype AG, 15 and AA 26  and control group show GG genotype 4 in comparison with other genotype AG 11, and AA 36 . Conclusions: The study is showed a high rate of chronic otitis media in patients with the GG genotype, suggesting that this genotype may be a risk factor compared to the control  group. On the other hand, the AA genotype was found to be ӏow in patients but high in the control group, indicating a possible is protected factor.