The role of IL-4 −590 (C>T) Gene as diagnostic biomarker of Hashimoto thyroiditis disease patients in AL-Najaf provenance /Iraq

Abstract

     The study of IL-4 genotyping in Hashimoto patients is considered one of the challenges of sustainable development .Hashimoto disease is an autoimmune disease that causes the immune system to attack the cells of the thyroid gland, causing the death of the cells that produce thyroid hormones, and thus leads to chronic hypothyroidism. The aim of current study is to determine the genotyping of IL-4 -590C>T in the DNA of Hashimoto patients and compared with healthy subjects .A 54 blood samples were collected from people with Hashimoto's disease and 25 samples from healthy people during period from March to September of the year 2022  , DNA was extracted from blood using Favorprep  Kit, then, using a specific primers for the C and T alleles of interleukin 4-590 C/T, then doubled using Arms PCR. Then, they were transported in agarose gel with the ethidium bromide dye and detected by the gel documentation system , ultraviolet rays use  to determine the type of alleles present in each sample and compared with control group. The current study, which includes 50/54 female and 4/54 male with Hashimoto thyroiditis with ages ranged from 20-59 years old, the results show up that the age group 40-49 years were the most affected, Also the body mass index was had increases among patients. CT genotype present in 74% of Hashimoto patients while 16% of control. C allele was found as high rate in both patients and control with percentage 100% and 96% respectively.  in conclusion: The Hashimoto patients have more frequency of CT genotyping of IL-4 -590 C>T than control ,while C Allele was high in both Hashimoto patients and controls. The -590 CC genotype in the IL4 gene may consider to be a strong predictive factor for the development of hypothyroidism in HD.