Measure the levels of TGF-β in Induced Wound Infection with Staphylococcus aureus

Abstract

Aim: Staphylococcus aureus wound infections are the leading cause of mortality and morbidity in patients around the world, Animal models is standard tools for studying a wide range of traumatic wound infections. The present paper is aim to study the effect of Staphylococcus aureus (SA) in wound and how the innate immune system interacts with the  infection , by Measure TGF-β cytokine , and there’s roles on recovery of wound. Methodology : 51 female  albino Rats  were used at the age of  ( 6-9) week , the animals were divided in to three group (17 animal in the group) the healthy control (Group I), wound control group(Group II), and infection contamination group with SA (Group III), , To mimic clinically occurring infections caused by SA infection , excision wound were made on dorsal side of the animals , and SA  2 × 108 (CFU)/mL were inoculated on the wound site in infection contamination group   ,  The blood collected after 24hours post infection for three days (24, 48, 72 hours) , histopathological examination with  ELISA tecinque was made for Measure TGF-β. Result :Immunological assay results of this study showed that the serum level for TGF-beta were  statistical significant between different values at different time when  (P ≤ 0.02). Conclusion: TGF-β is a potent immunomodulatory that initiates and terminates tissue repair. It is released in wound areas after tissue damage by the inflammatory cell .