Evaluation trace minerals and oxidative status in follicular fluid for unexplained infertile female undergoing Intra-cytoplasmic sperm injection program.

Abstract

Objective: To evaluate the level of zinc and 8-OHdg in follicular fluid and determine the correlation of both with Intracytoplasmic sperm injection outcome.Aim: The aim of this study is. To evaluate the Level of zinc and oxidative stress biomarker 8-OHdg in follicular fluid for unexplained female and compare with male factor. Study the correlation between zinc and 8-OHdg with ICSI outcome. Material and method: eighty eight subfertile couples were involved in this study. They divided into two groups. First group male factor and second group unexplained female factor. Both were interred in ICSI program. Result: The study showed that the mean follicular fluid level of zinc in pregnant group (17.47) and non-pregnant group (16.62). However, not different level of zinc in follicular fluid in both group unexplained female factor and male factor (16.66) and (16.97), respectively. The level of zinc significantly with No. of the oocyte (p=0.004), MII (p=0.033), No. of 2PN (p=0.035), Fertilization rate (p=0.001), Embryo G1 (p=0.044) and Embryo G2 (p=0.026). No significant statistical difference between level of 8- OHdg in follicular fluid in two groups regarding the cause of infertility unexplained female factor and male factor mean (2.20) and (2.25), respectively. But significant with pregnant status in pregnant group mean (1.37) and non pregnant group mean (2.54). The level of 8-OHdg non-significantly with ICSIoutcome. Conclusion: zinc level is statically significant and positively correlated with ICSI outcome, as the number of oocytes, mature oocyte (MII), fertilized oocytes 2PN, fertility rate, embryo G1 and embryo G2.The level of 8-OHdg in follicular fluid statically non- significant with ICSI outcome and not found a correlation between zinc and 8-OHdg.