Molecular identification and Virulence factors of Pseudomonas aeruginosa isolated from operation hall

Abstract

This study aimed to determine the P.aeruginosa that causes contamination to operation hall in hospitals by detecting of pathogenisity markers ..Moreover, the virulence factors  of predominant species were detected phenotypically  by using   routine  techniques, that responsible for pathogenicity.  Fifteen  samples were collected from different sites of operation hall during  two months  2022 in AL-Hussein  hospital/ karbala City. The identification of P.aeruginosa isolates depended on colonial morphology, microscopic examination and biochemical tests as a primary identification. The final identification was confirm by PCR  technique from different sites  .The  results  obtained  by  the  PCR  tests were twenty two isolates of P.aeruginosa  were  detected , which  distributed  into :(9) earth, (8) wall, and (10) beds. The  study  investigated  the  virulence  factors  of  P.aeruginosa, which  had  the  ability  to  produce capsule, biofilm , adhesion ,protease, bacteriocin ,haemolysin and   β-lactamase  and gelatinase .             The  results  revealed  variation  in  the  resistance of bacteria to some antibiotics,..P.aeruginosa exhibited high resistance (96%) to Cefotaxime, but absolute susceptibility to Ciproflaxacin and Gentamycin and high susceptibility to Amikacin.Ceftiaxone,and Azithromycin.