Abstract
Cancer is a serious public health problem, and one of the main causes of cervical cancer in women is the infection with high/risk Human papillomavirus (HPV). Human papillomaviruses are DNA viruses that have specific tropism for squamous epithelia. More than 120 different HPV types have been isolated to date; of these Low-risk HPV types, such as HPV6 and HPV11, induce benign hyperproliferations of the epithelium such as papilloma or warts. By contrast, high-risk oncogenic types (HPV16, -18, -31, -33, -35, -39, -45, - 52, - 56, - 58, - 59, - 66) are defined to have strong epidemiologic association with cervical cancer. Multiplex-PCR analysis was used for the detection and genotyping of high/risk HPV DNA in the cytological samples of cervical squamous intraepithelial lesions.The results show that the proportion of High/risk HPV genotypes in cervical smears of patients with cervical squamous intraepithelial neoplasia was 59%. In details, the genotypes were: HPV16 (15.3%), HPV18 (15.3%), HPV33 (7.7%), HPV31 (7.7%), HPV35 (30.7%), HPV56 (7.7%), HPV45 (7.7%), HPV58 (7.7%) and HPV52 (7.7%). In the other hand, none of the DNA isolated from 10 healthy group showed high/risk HPV