The Protective Effect of Vanadium on Cyclophosphamide-Induced Teratogenesis in Mouse Fetus

Abstract

Cyclophosphamide(CP) is a chemotherapeutic and immunosuppressive drug used for treatment of neoplastic and some auto-immune diseases, but it has several important adverse effects and can induce external malformations in the fetus. Different materials can use to avoid or prevent its side effects. Trace elements as an antioxidant can prevent oxidative stress by binding to free radicals and reduce adverse effects of chemotherapy drugs. vanadium is present as an ultratrace element has prophylactic effects on teratogenic effects of CP. The aim of our work was to estimate the vanadium effect on CP-induced macroscopic fetal defects in mice. In this study pregnant NMRI mice were divided into four groups. control group received normal saline, CP group received CP (10 mg/kg on 11th GD), V group received vanadium (4mg/kg on 8th,10th and 12th) and V/CP group CP (10 mg/kg on 11th GD) with vanadium (4mg/kg on 8th,10th and 12th) intraperitoneally. Fetuses were collected on the 19th GD. Then the weight of fetuses, length of crown-rump, limbs, and tail of fetuses were measured. The external teratogenesis were investigated by the stereomicroscope.