Affiliation:
1. Department of Parasitology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia;
2. Vector Borne Disease Sector, Ministry of Health, Putrajaya, Malaysia
Abstract
ABSTRACT.
This study highlights the development of two lateral flow recombinase polymerase amplification assays for the diagnosis of human malaria. The lateral flow cassettes contained test lines that captured biotin-, 6-carboxyfluorescein, digoxigenin-, cyanine 5-, and dinitrophenyl-labeled amplicons. The overall process can be completed in 30 minutes. Recombinase polymerase amplification coupled with lateral flow had a detection limit of 1 copy/µL for Plasmodium knowlesi, Plasmodium vivax, and Plasmodium falciparum. No cross-reactivity was observed among nonhuman malaria parasites such as Plasmodium coatneyi, Plasmodium cynomolgi, Plasmodium brasilanium, Plasmodium inui, Plasmodium fragile, Toxoplasma gondii, Sarcocystis spp., Brugia spp., and 20 healthy donors. It is rapid, highly sensitive, robust, and easy to use. The result can be read without the need for special equipment and thus has the potential to serve as an effective alternative to polymerase chain reaction methods for the diagnosis of malaria.
Publisher
American Society of Tropical Medicine and Hygiene
Subject
Virology,Infectious Diseases,Parasitology
Reference36 articles.
1. World Malaria Report 2021,2021
2. DNA detection using recombination proteins;Piepenburg,2006
3. Rapid detection of Plasmodium falciparum with isothermal recombinase polymerase amplification and lateral flow analysis;Kersting,2014
4. Bushmeat species identification: recombinase polymerase amplification (RPA) combined with lateral flow (LF) strip for identification of Formosan Reeves’ Muntjac (Muntiacus reevesi micrurus);Hsu,2021
5. Rapid detection of the pathogenic fungi causing blackleg of Brassica napus using a portable real-time fluorescence detector;Lei,2019