Detection of Human Paragonimiasis by ELISA Using Recombinant Paragonimus westermani Cysteine Protease 7-Reference-Cited by-同舟云学术

Detection of Human Paragonimiasis by ELISA Using Recombinant Paragonimus westermani Cysteine Protease 7

Published:2023-07-05 Issue:1 Volume:109 Page:166-169
ISSN:0002-9637
Container-title:The American Journal of Tropical Medicine and Hygiene
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Author:

Andrade-Gomes Luana Gabriele¹,Zuniga María J.¹,Dolz Gaby¹²,Solano-Campos Frank³

Affiliation:

1. Maestría en Enfermedades Tropicales, Posgrado Regional en Ciencias Veterinarias Tropicales, Universidad Nacional, Heredia, Costa Rica;

2. Laboratorio de Zoonosis y Entomología, Programa de Medicina Poblacional, Escuela de Medicina Veterinaria, Universidad Nacional, Heredia, Costa Rica;

3. Escuela de Ciencias Biológicas, Universidad Nacional, Heredia, Costa Rica

Abstract

ABSTRACT. Paragonimiasis is an important but neglected foodborne trematodiasis caused by Paragonimus mexicanus in Costa Rica. Immunological techniques for diagnosing this parasitosis in humans do not exist in Central America. The objective of the present study was to use recombinant Paragonimus westermani cysteine protease 7 to standardize an ELISA for the detection of antibodies against Paragonimus spp. Human sera positive for P. westermani, P. mexicanus, or Paragonimus spp., human sera infected with other helminths, as well as sera of healthy humans without parasitic infections, were analyzed. The sensitivity of the ELISA was 92.9%, and the specificity was 91.9%. This report is the first to describe the development of an ELISA for the diagnosis of Paragonimus spp. in Costa Rica and Central America. Using this ELISA in the health system of Costa Rica is recommended to detect infections.

Publisher

American Society of Tropical Medicine and Hygiene

Subject

Virology,Infectious Diseases,Parasitology

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