Real-Time Polymerase Chain Reaction Method for the Detection of Onchocerca volvulus in Post-Elimination Surveillance of Onchocerciasis in Ecuador-Reference-Cited by-同舟云学术

Real-Time Polymerase Chain Reaction Method for the Detection of Onchocerca volvulus in Post-Elimination Surveillance of Onchocerciasis in Ecuador

Published:2023-12-06 Issue:6 Volume:109 Page:1368-1371
ISSN:0002-9637
Container-title:The American Journal of Tropical Medicine and Hygiene
language:
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Author:

Salazar Esteban¹²³,Morales Diego⁴,Febrer-Sendra Begoña³,Fernández-Soto Pedro³,López-Abán Julio³,Quinatoa Paul⁴,Guevara Ángel¹,Muro Antonio³

Affiliation:

1. Instituto de Biomedicina, Carrera de Medicina, Universidad Central, Quito, Ecuador;

2. Distrito 17D03 Ministerio de Salud Pública del Ecuador, Quito, Ecuador;

3. Infectious and Tropical Diseases Research Group, Biomedical Research Institute of Salamanca Research Centre for Tropical Diseases at the University of Salamanca, Faculty of Pharmacy, University of Salamanca, Salamanca, Spain;

4. Instituto Nacional de Investigación en Salud Pública Dr. Leopoldo Izquieta Pérez, Quito, Ecuador

Abstract

ABSTRACT. Onchocerciasis has been declared eliminated in Ecuador and surveillance measures are of great interest. In this study, we examined the infectivity rates of Simulium exiguum by Onchocerca volvulus in previously hyperendemic areas in Esmeraldas province of Ecuador. These areas had previously undergone mass administration of ivermectin, which led to the interruption of transmission in 2009 and the certification of elimination in 2014. The study included three communities in Río Cayapas and one in Río Canandé, and a total of 2,950 adult S. exiguum were collected in 2018. We used quantitative polymerase chain reaction with O. volvulus O-150 plasmid control DNA to analyze 59 pools. Our findings revealed that the infectivity rates were zero, indicating that the transmission of O. volvulus remained suspended in the area.

Publisher

American Society of Tropical Medicine and Hygiene

Subject

Virology,Infectious Diseases,Parasitology

Link

https://www.ajtmh.org/downloadpdf/journals/tpmd/109/6/article-p1368.xml

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