Ventral Approach to the Rat Middle Ear for Otologic Research

Author:

Pinilla Mayte12,Ramírez-Camacho Rafael12,Jorge Eduardo13,Trinidad Almudena12,Vergara José12

Affiliation:

1. Madrid, Spain

2. Departments of Otorhinolaryngology

3. Experimental Surgery, Clínica Puerta de Hierro. Universidad Autónoma.

Abstract

OBJECTIVE: To describe a simple approach to the rat middle ear because there are few clear descriptions of this technique with minimal morbidity and mortality. STUDY DESIGN AND SETTING: Six male Long-Evans rats were used in this study. We designed a cervical approach to the middle ear with the purpose of creating an experimental model of ossiculoplasty that permits the postoperative survival of the animal. RESULTS: It was possible in all cases to visualize the majority of structures of the tympanic cavity: promontory, round window, stapedial artery, stapes, incus, and tympanic membrane. There were no cases of postoperative infection or facial paralysis. CONCLUSIONS AND SIGNIFICANCE: The ventral approach to the rat middle ear is a rapid and simple technique that makes the rat the animal of choice for many otologic investigations.

Publisher

SAGE Publications

Subject

Otorhinolaryngology,Surgery

Reference8 articles.

1. Surgical anatomy of the rat middle ear

2. 2. Rudolf Hebel MW, Stromberg L. Sensory organs In Anatomy of the laboratory rat. Baltimore: Waverly Press; 1976. p. 146–52.

3. Surgical Anatomy of the Guinea Pig Ear

4. 5. Anson BJ, Donaldson JA. The middle ear In Surgical anatomy of the temporal bone and ear, 2nd ed. Philadelphia: WB Saunders Co; 1981. p. 31–42.

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