Carbon-Electrode Dielectrophoresis for Bioparticle Manipulation

Abstract

This work presents the latest application of carbon-electrode Dielectrophoresis, a technique for particle separation. The use of 3D carbon electrodes allows for higher throughput and efficiency when compared to more traditional DEP techniques, such as metal-electrode and insulator-based DEP. The applications presented here all feature a protocol that allows for enrichment and purification of a targeted particle population from a sample; and effectively enables sample preparation for downstream analysis. The final goal is the integration of independent carbon-electrode arrays, optimized in their geometry and polarizing signals, to achieve an automated sample preparation device comprising functions such as concentration, purification and lysis.