Abstract
Recent advances in electrochemical biosensors have focused on new materials and strategies to improve specificity, sensitivity, stability, and response time. Herein, we aim to develop an electrochemical biosensor device by modification of a screen-printed electrode (SPE) with highly porous Au nanostructures and a bioluminescence (BL)-producing enzyme (luciferase). This approach leverages the enhanced electrochemically active surface area and the mass transport effect and offers an alternative configuration for optical output from the enzyme. The BL presents an instantaneous measurement of enzyme activity and can be exploited to show that the enzyme is being electrochemically controlled (an ON-OFF switchable sensor).
Publisher
The Electrochemical Society