Abstract
A cobalt metal-organic framework (Co MOF)-ionic liquid (IL) nanocomposite comprising the base matrix of the electrode was developed with electropolymerized molecularly imprinted polymer (MIP) consisting of o-phenylenediamine (oPD) to detect carcinoembryonic antigen (CEA), a biomarker of breast cancer. Firstly, Co MOF was synthesized using hydrothermal synthesis, which was used as an electrically conductive skeleton and functionalized with an IL. Then, CEA was employed as a template in the electropolymerization of oPD-imprinted films on the surface of screen-printed carbon electrode (SPCE). Finally, the template protein was removed to form a molecularly imprinted film capable of qualitatively and quantitatively signaling CEA. Under optimized conditions, the sensor for CEA exhibits a wide detection range of 1.0 × 10−4−10 ng·ml−1 (0.32 V vs Ag/AgCl) with a limit of detection (LOD) and limit of quantification (LOQ) of 0.024 pg·ml−1 and 0.082 pg·ml−1, respectively. Besides, other parameters including the selectivity, reproducibility (RSD 2.2%), and recovery rate (92.16%–103.35%) are all satisfactory. The proposed strategy provides a new route for the development of rapid breast cancer diagnostic tools.
Funder
the National Key R&D Program of China
Publisher
The Electrochemical Society
Subject
Materials Chemistry,Electrochemistry,Surfaces, Coatings and Films,Condensed Matter Physics,Renewable Energy, Sustainability and the Environment,Electronic, Optical and Magnetic Materials
Cited by
6 articles.
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