An Acetylcholine Electrochemical Biosensor Based on Bi-Enzyme Functionalized Nanofiber Composites

Author:

Jing Chunyang,Kuang Yijing,Gu Xuan,Xu Mingming,Wu Yuke,Wang XiaoyingORCID

Abstract

This work offered an electrochemical biosensor for acetylcholine (ACh) detection using the bi-enzyme functionalized nanofibers composites-modified glassy carbon electrode (GE). The nanofibers composites (AuNPs@GCS) prepared by electrospinning and electrodeposition techniques, were systematically characterized from micromorphology, active groups, actual areas and impedance, and were used to simultaneously decorate the acetylcholinesterase (AChE) and choline oxidase (ChOx). Bi-enzyme functionalized AuNPs@GCS (AChE-ChOx/AuNPs@GCS) electrode demonstrated the superior loading capacity and stability, was firstly used for the ACh analysis, the detection limit was 0.067 nM, it outperformed or compared to the relevant literatures in terms of linear range and LOD. After systematic methodological evaluation and feasibility verification, this biosensor had good stability, specificity and reproducibility. In human serum samples, the results detected by the biosensor were compared with results tested by Enzyme-linked immunosorbent assay (ELISA). T test was performed for statistical comparisons and p value more than 0.05 was considered no statistical significance. It indicated that this biosensor had a good application prospect in the ACh actual sample detection. This work also provided an efficient and accurate method for quantitative detection of neurotransmitters in complex biological samples.

Publisher

The Electrochemical Society

Subject

Materials Chemistry,Electrochemistry,Surfaces, Coatings and Films,Condensed Matter Physics,Renewable Energy, Sustainability and the Environment,Electronic, Optical and Magnetic Materials

Cited by 3 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3