Abstract
A label-free molecularly imprinted electrochemical sensor (MIECS) based on electropolymerized molecularly imprinted polymer (MIP) was developed for the determination of homocysteine (Hcy) in serum for the first time. MXene@AuNPs with layered structure was synthesized on the electrode by modifying MXene with gold nanoparticles (AuNPs). MIP based on dopamine hydrochloride (DA) were electropolymerized onto the surface of MXene@AuNPs modified electrode by molecular docking and quantum chemical calculations for specific recognition of Hcy. MXene@AuNPs as a carrier for immobilizing MIP steadily enhances the conductivity of the electrode (about 4.2-fold) and plays a crucial part in improving the detection sensitivity of MIECS. The results showed that the current response linearly decreased with the increasing concentration of Hcy in the detection range from 1 × 10−13 to 1 × 10−5 mol l−1, the limit of detection (LOD) and limit of quantification (LOQ) of 11.81 fmol l−1 and 39.49 fmol l−1, respectively. With favorable selectivity, stability, reproducibility and ruggedness, the developed MIECS was applied to the determination of Hcy in human serum samples with recoveries of 87.83%–92.58%. The proposed strategy has potential application for disease surveillance.
Funder
the National Key R&D Program of China
Publisher
The Electrochemical Society
Subject
Materials Chemistry,Electrochemistry,Surfaces, Coatings and Films,Condensed Matter Physics,Renewable Energy, Sustainability and the Environment,Electronic, Optical and Magnetic Materials
Cited by
2 articles.
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