Modeling Electropolymerization of Methylene Blue in the Presence of Pillar[6]arene and DNA

Abstract

For the first time, cumulative effect of the changes in the concentration of methylene blue (MB), pillar[6]arene (P6) and DNA in the MB electropolymerization performed by repeated cycling of the potential of the glassy carbon electrode (GCE) modified with carbon black (CB) was described by construction of generalized linear models within the full factor experiment design. The proposed models of the currents and potentials of the peaks attributed to the monomeric and polymeric MB forms fully describe synergistic effect of the reaction mixture components and their contribution to the efficiency of electropolymerization and accumulation of the redox active layer on the electrode. The MB concentration exerted the biggest effect on the redox peak currents of the coating. DNA addition decreased the redox peak currents to a degree mostly pronounced for the high MB concentrations. The P6 addition significantly improved the conditions of the electron exchange and increased the redox peak currents, especially at low MB concentrations. The results obtained can find application for the optimization of the assembling conditions in the DNA sensors design.