Abstract
A simple and sensitive electrochemical sensor was developed to quantify ciprofloxacin by restricted electro-polymerization of aniline on GCE followed by drop coating functionalized MWCNTs. Electro-catalytic activity of modified electrodes was investigated by EIS and CV revealed decrease in electrode’s charge transfer resistance and increase in electron transfer kinetics. Effect of pH and scan rate suggests a mixed adsorption-diffusion process. Tafel slope (plot of ln∣j
a
∣ or ln∣I
a
∣ against E) with linear relationship of potential vs logarithm of current on the linear sweep voltammogram determined the electron transfer coefficient (α). Using the value of (α), number of electrons (n) involved in the rate determining step was obtained. Surface coverage of analyte molecules adsorbed, and diffusion coefficient of ciprofloxacin were estimated from the Laviron and Randles Sevcik equations respectively. Peak current obtained by LSV for various concentrations of ciprofloxacin exhibited two linear ranges, 0.1 μM to 1 μM and 1 μM to 20 μM, with limit of detection 0.08 μM (RSD = 2.4%, S/N = 3). Presence of species existing abundantly in the sample matrix do not affect the sensor signal. Proposed ciprofloxacin sensor demonstrated high reproducibility, long-term stability and fast reaction. The fabricated sensor successfully determined ciprofloxacin in pharmaceutical formulations with recoveries between 92 to 104%.
Publisher
The Electrochemical Society
Subject
Materials Chemistry,Electrochemistry,Surfaces, Coatings and Films,Condensed Matter Physics,Renewable Energy, Sustainability and the Environment,Electronic, Optical and Magnetic Materials
Reference77 articles.
1. Fluoroquinolones: mechanism of action, classification and development of resistance;Blondeau;Survey of ophthalmology,2004
2. New fluoroquinolone antibiotics;Activity;Drug and Therapeutics Bulletin,1999
3. Quinolones: A comprehensive review;Oliphant;Am Fam Physician.,2002
4. Quinolones: Action and resistance updated;Drlica;Curr. Top. Med. Chem.,2009
5. Mechanisms of action of antimicrobials: focus on fluoroquinolones;Hooper;Clin. Infect, Dis.,2001
Cited by
7 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献