<p>In order to study the chemical parameters of the soil after sub-irrigation with wastewater, a system was installed in one of the greenhouses of the Agricultural University of Athens. Wastewater was applied subsurface into the soil mass of the pots were used. Three treatments were used: Untreated wastewater (U), Treated wastewater (T) and tap water (W) as control. Two different types were used: Soil (a) characterized as Sandy loam and soil (b) characterized as Loamy sand. Moreover, in order to investigate the change of total Nitrogen and organic matter concentrations at the point where the emitter was placed, the soil mass was divided into two zones. The upper (zone I) and the lower one (zone II). The total nitrogen content, ammonia nitrogen (NH4-N), nitrogen nitrate (NO3-N) and the percentage of organic matter, were determined in the soil samples. Statistically significant differences (p <0, 05) were observed in the organic matter and the total N%, only for soil (b). For soil (a), organic matter percentage was increased in zone (I) (irrigation with treated wastewater at 20 cm depth). For soil (b), total N% was increased in zone (I), while nitrate and ammonium were increased in zone (II) (irrigation with untreated wastewater at 20 cm depth).</p>