Application of Several Special Staining Methods for Paraffin Sections on Epon-Embedded Semithin Sections

Author:

AKBAŞ Ayşe1ORCID,YAVAŞ Senem Esin2ORCID,ERSOY Semiha3ORCID,BAŞAR Doruk1ORCID

Affiliation:

1. BURSA ULUDAĞ ÜNİVERSİTESİ, TIP FAKÜLTESİ, TEMEL TIP BİLİMLERİ BÖLÜMÜ, HİSTOLOJİ VE EMBRİYOLOJİ ANABİLİM DALI

2. BURSA ULUDAĞ ÜNİVERSİTESİ, SAĞLIK BİLİMLERİ ENSTİTÜSÜ, HİSTOLOJİ-EMBRİYOLOJİ (DR)

3. Bursa Uludağ Üniversitesi Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Bursa.

Abstract

Aim: This study aimed to compare several specific staining protocols recommended for paraffin sections and toluidine blue and light green double staining combination to be tried for the first time with routine toluidine blue staining on semithin epon sections. Material and Methods: Samples of 1x1x1 mm were taken from the liver, skin, and aorta tissues of Wistar albino adult rats. Tissue samples were fixed with 5% glutaraldehyde at +4º C overnight, postfixed with 1% osmium tetroxide for one hour, and then, blocked with Epon 812 after processing. Semithin sections of 1 μm thickness were obtained from the epon blocks. Sections were stained with Altmann’s method (for mitochondria), Verhoeff’s method (for elastic fibers), Gordon&Sweets’ silver impregnation method (for type III collagen), toluidine blue and light green double staining combination (for type I collagen) and routine toluidine blue method. Results: In liver sections, mitochondria in hepatocytes were differentiated by the Altmann method, and stromal type III collagen fibers were distinguished with Gordon&Sweets’ method. Elastic lamellar structures were easily observed in black in the aortic sections stained with the Verhoeff method. Successful results were obtained in the staining of dermal type I collagen with toluidine blue and light green double staining in skin sections. Conclusion: Since the specific staining tried for the first time gave positive results in epon sections, it was concluded that these methods can be used to determine the localization of cellular and intercellular components that are aimed to be examined at the ultrastructural level.

Publisher

Duzce Medical Journal

Subject

General Medicine

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