Random mutagenesis and screening of complex glycoproteins: expression of human gonadotropins in Dictyostelium discoideum
Author:
Affiliation:
1. Cell Engineering Facility GBBUniversity of Groningen Nijenborgh 4 9747 AG Groningen The Netherlands
2. N.V. Organon P.O. Box 20 5340 BH Oss The Netherlands
Publisher
Wiley
Subject
Genetics,Molecular Biology,Biochemistry,Biotechnology
Link
https://onlinelibrary.wiley.com/doi/pdf/10.1096/fasebj.13.6.639
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2. Plunkett M. J. andEllman J. A.(1997)Combinatorial chemistry and new drugs.Sci. Am.55–59
3. Recombinant technique and gonadotropins production: new era in reproductive medicine
4. Molecular Basis of the Specificity of Binding of Glycoprotein Hormones to Their Receptors
5. Priority paper Partially deglycosylated human choriogonadotropin, stabilized by intersubunit disulfide bonds, shows full bioactivity
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1. Dictyostelium discoideum—a promising expression system for the production of eukaryotic proteins;The FASEB Journal;2008-08-19
2. Production and characterization of pharmacologically active recombinant human phosphodiesterase 4B inDictyostelium discoideum;Biotechnology Journal;2008-07
3. High-level expression of biologically active glycoprotein hormones in Pichia pastoris strains—selection of strain GS115, and not X-33, for the production of biologically active N-glycosylated 15N-labeled phCG;Glycoconjugate Journal;2008-02-15
4. Desalted Deep Sea Water Increases Transformation and Homologous Recombination Efficiencies in Dictyostelium discoideum;Journal of Molecular Microbiology and Biotechnology;2008
5. Regulation of Ammonia Homeostasis by the Ammonium Transporter AmtA in Dictyostelium discoideum;Eukaryotic Cell;2007-12
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