Unraveling the role of lipid droplets and perilipin 2 in bovine luteal cells

Author:

Plewes Michele R.123ORCID,Talbott Heather A.1ORCID,Schott Micah B.3ORCID,Wood Jennifer R.4ORCID,Cupp Andrea S.4ORCID,Davis John S.123ORCID

Affiliation:

1. Olson Center for Women's Health, Department of Obstetrics and Gynecology University of Nebraska Medical Center Omaha Nebraska USA

2. Veterans Affairs Nebraska Western Iowa Health Care System Omaha Nebraska USA

3. Department of Biochemistry and Molecular Biology University of Nebraska Medical Center Omaha Nebraska USA

4. Department of Animal Sciences University of Nebraska–Lincoln Lincoln Nebraska USA

Abstract

AbstractSteroidogenic tissues contain cytosolic lipid droplets that are important for steroidogenesis. Perilipin 2 (PLIN2), a structural coat protein located on the surface of lipid droplets in mammalian cells, plays a crucial role in regulating lipid droplet formation and contributing to various cellular processes such as lipid storage and energy homeostasis. Herein, we examine the role that PLIN2 plays in regulating progesterone synthesis in the bovine corpus luteum. Utilizing gene array databases and Western blotting, we have delineated the expression pattern of PLIN2 throughout the follicular to luteal transition. Our findings reveal the presence of PLIN2 in both ovarian follicular and steroidogenic luteal cells, demonstrating an increase in its levels as follicular cells transition into the luteal phase. Moreover, the depletion of PLIN2 via siRNA enhanced progesterone production in small luteal cells, whereas adenovirus‐mediated overexpression of both PLIN2 and Perilipin 3 (PLIN3) induced an increase in cytosolic lipid droplet accumulation and decreased hormone‐induced progesterone synthesis in these cells. Lastly, in vivo administration of the luteolytic hormone prostaglandin F2α resulted in an upregulation of PLIN2 mRNA and protein expression, accompanied by a decline in serum progesterone. Our findings highlight the pivotal role of PLIN2 in regulating progesterone synthesis in the bovine corpus luteum, as supported by its dynamic expression pattern during the follicular to luteal transition and its responsiveness to luteotropic and luteolytic hormones. We suggest PLIN2 as a potential therapeutic target for modulating luteal function.

Funder

U.S. Department of Agriculture

U.S. Department of Veterans Affairs

Eunice Kennedy Shriver National Institute of Child Health and Human Development

Publisher

Wiley

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