Effect of Resveratrol as Caloric Restriction Mimetic and Environmental Enrichment on Body Weight, Lipid Profile and Electrolytes in Young Healthy Mice

Abstract

Caloric restriction (CR) and Environmental Enrichment have been separately reported to possess health benefits such as protection against obesity, cancer, cardiovascular and neurodegenerative diseases. Resveratrol, a natural polyphenolic compound has been reported to be caloric restriction mimetic. The aim of this study was to investigate the potential benefit of the combination of Resveratrol as CR mimetic and EE on Lipid Profile in young healthy mice. Fifty mice of both sexes were randomly divided into five groups of 10 animals each: group I served as control, group II were maintained on alternate day feeding (ADF), group III received Resveratrol 50 mg/kg, suspended in 10 g/L of (CMC) orally per kg/day. Group IV received CMC and kept in an Enriched Environment (EE) while group V received Resveratrol 50 mg/kg and kept in EE. The treatment lasted for 28 days. The animals were sacrificed 24 hours after the last treatment and the plasma sample was collected for biochemical evaluation. The results obtained showed significant decrease (P < 0.05) in high density lipoprotein concentration in ALDF group when compared to the control. A significant increase (P < 0.05) was also observed in low density lipoprotein concentration in ADF group when compared to the control. There was no significant change observed in the body weight and electrolyte contents of the animals during the study period. In conclusion, the results obtained indicates that Resveratrol as CR mimetic and EE have no significant effects on body weight, lipid profile and electrolyte contents in young healthy mice over a period of four weeks. Effect of Resveratrol‐induced CR and EE on lipid profiles in mice Groups TC (mg/dl) TG (mg/dl) HDL (mg/dl) LDL (mg/dl) Control (CMC) 2.38 ± 0.10 0.86 ± 0.08 1.088 ± 0.08 1.12 ± 0.05 ALDF 2.45 ± 0.07 0.94 ± 0.07 0.70 ± 0.06* 1.56 ± 0.10* Resveratrol only 2.23 ± 0.07 0.88 ± 0.10 0.75 ± 0.08 1.30 ± 0.07 EE only 2.38 ± 0.08 0.96 ± 0.05 0.76 ± 0.10 1.48 ± 0.16 Resveratrol + EE 2.51 ± 07 0.80 ± 0.09 0.87 ± 0.09 1.48 ± 0.08 P<0.05, CMC = Carboxymethyl‐cellulose, ALDF = Alternate day feeding, EE = Environmental Triacylglycerol, HDL = High density lipoprotein, LDL = Low density lipoprotein Effect of Resveratrol induced CR and EE on Electrolyte content in mice Groups Na (mmol/L) K (mmol/L) Cl (mmol/L) Control (CMC) 139.75 ± 0.92 4.04 ± 0.11 99.75 ± 1.88 EODF 139.30 ± 0.86 4.17 ± 0.11 100.70 ± 0.93 Resveratrol only 129.60 ± 10.42 4.02 ± 0.15 100.70 ± 1.13 EE only 139.00 ± 1.21 4.11 ± 0.18 100.13 ± 1.29 Resveratrol + EE 137.30 ± 1.28 4.33 ± 0.09 99.60 ± 1.09 * P < 0.05, CMC = Carboxymethyl‐cellulose, ALDF = Alternate day feeding, EE = Environmental enrichment, Na = Sodium, K = Potassium, Cl = Chloride