Age‐related flexibility of energetic metabolism in the honey bee Apis mellifera

Author:

Menail Hichem A.12ORCID,Cormier Simon B.12,Léger Adèle12,Robichaud Samuel12,Hebert‐Chatelain Etienne13ORCID,Lamarre Simon G.3ORCID,Pichaud Nicolas12ORCID

Affiliation:

1. New Brunswick Centre for Precision Medicine Moncton New Brunswick Canada

2. Department of Chemistry and Biochemistry Université de Moncton Moncton New Brunswick Canada

3. Department of Biology Université de Moncton Moncton New Brunswick Canada

Abstract

AbstractThe mechanisms that underpin aging are still elusive. In this study, we suggest that the ability of mitochondria to oxidize different substrates, which is known as metabolic flexibility, is involved in this process. To verify our hypothesis, we used honey bees (Apis mellifera carnica) at different ages, to assess mitochondrial oxygen consumption and enzymatic activities of key enzymes of the energetic metabolism as well as ATP5A1 content (subunit of ATP synthase) and adenylic energy charge (AEC). We also measured mRNA abundance of genes involved in mitochondrial functions and the antioxidant system. Our results demonstrated that mitochondrial respiration increased with age and favored respiration through complexes I and II of the electron transport system (ETS) while glycerol‐3‐phosphate (G3P) oxidation was relatively decreased. In addition, glycolytic, tricarboxylic acid cycle and ETS enzymatic activities increased, which was associated with higher ATP5A1 content and AEC. Furthermore, we detected an early decrease in the mRNA abundance of subunits of NADH ubiquinone oxidoreductase subunit B2 (NDUFB2, complex I), mitochondrial cytochrome b (CYTB, complex III) of the ETS as well as superoxide dismutase 1 and a later decrease for vitellogenin, catalase and mitochondrial cytochrome c oxidase subunit 1 (COX1, complex IV). Thus, our study suggests that the energetic metabolism is optimized with aging in honey bees, mainly through quantitative and qualitative mitochondrial changes, rather than showing signs of senescence. Moreover, aging modulated metabolic flexibility, which might reflect an underpinning mechanism that explains lifespan disparities between the different castes of worker bees.

Funder

Natural Sciences and Engineering Research Council of Canada

New Brunswick Innovation Foundation

Publisher

Wiley

Subject

Genetics,Molecular Biology,Biochemistry,Biotechnology

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