Affiliation:
1. Applied Science and Technology North Carolina A&T State University Greensboro North Carolina USA
2. Department of Electrical and Computer Engineering North Carolina A&T State University Greensboro North Carolina USA
3. Department of Computer Systems Technology North Carolina A&T State University Greensboro North Carolina USA
4. Department of Biology North Carolina A&T State University Greensboro North Carolina USA
Abstract
AbstractThe Ebola virus poses a severe public health threat, yet understanding factors influencing disease outcomes remains incomplete. Our study aimed to identify critical pathways and hub genes associated with fatal and survivor Ebola disease outcomes. We analyzed differentially expressed hub genes (DEGs) between groups with fatal and survival outcomes, as well as a healthy control group. We conducted additional analysis to determine the functions and pathways associated with these DEGs. We found 13,198 DEGs in the fatal and 12,039 DEGs in the survival group compared to healthy controls, and 1873 DEGs in the acute fatal and survivor groups comparison. Upregulated DEGs in the comparison between the acute fatal and survivor groups were linked to ECM receptor interaction, complement and coagulation cascades, and PI3K‐Akt signaling. Upregulated hub genes identified from the acute fatal and survivor comparison (FGB, C1QA, SERPINF2, PLAT, C9, SERPINE1, F3, VWF) were enriched in complement and coagulation cascades; the downregulated hub genes (IL1B, 1L17RE, XCL1, CXCL6, CCL4, CD8A, CD8B, CD3D) were associated with immune cell processes. Hub genes CCL2 and F2 were unique to fatal outcomes, while CXCL1, HIST1H4F, and IL1A were upregulated hub genes unique to survival outcomes compared to healthy controls. Our results demonstrate for the first time the association of EVD outcomes to specific hub genes and their associated pathways and biological processes. The identified hub genes and pathways could help better elucidate Ebola disease pathogenesis and contribute to the development of targeted interventions and personalized treatment for distinct EVD outcomes.
Reference75 articles.
1. World Health Organization.Ebola virus disease.www.who.intWeb site.https://www.who.int/news‐room/fact‐sheets/detail/ebola‐virus‐disease. Updated 2023. Accessed January 2023
2. Ebola haemorrhagic fever
3. Ebola virus disease: A review for the emergency medicine clinician
4. Ebola virus disease
5. Ebola virus disease