Study of antigenic and immunogenic activity of experimental series of inactivated emulsified vaccine against Chicken hemophilosis in laboratory conditions

Abstract

Worldwide, Avibacterium paragallinarum is the etiological agent of infectious rhinitis in poultry. Most vaccines are based on international reference strains without taking into account the current epizootic situation of hemophilosis in the respective territories. Using a mixture of inactivated Av. paragallinarum antigens (SS 6/20, A; SS 7/20, B; SS 8/20, C) of three serotypes (1:1:1) and (sample No 1 – AG + «aluminum hydroxide»; No 2 – AG + «aluminum hydroxide + saponin»; No 3 – AG + «Montanide ISA 70») obtained experimental samples of the domestic inactivated vaccine against chicken rhinitis. The antigenic and immunogenic activity of the inactivated trivalent vaccine on chickens was established. A commercial vaccine registered on the territory of Ukraine was used as a comparison. Vaccination of birds was carried out twice in a dose of 0.5 cm3 with an interval of 21 days, subcutaneously, in the area of the middle third of the neck. The investigated experimental samples No 2 and No 3 are not inferior to the commercial vaccine in terms of antigenic activity; antibody levels range from 1:64 to 1:512. The immunogenic activity of these samples is 80–100% compared to the commercial vaccine (poultry group 4), the immunogenic activity of which is at the same level. Replication of the causative agent of |Haemophilus infection in chickens from the paternal material of chickens vaccinated with a commercial vaccine (group 4) and experimental vaccines №2 (group 2, adjuvant – «aluminum hydroxide + saponin») and №3 (group 3, adjuvant – «Montanid ISA 70») and infected with control strains of the pathogen was not noted