Method of biochemical change corrections in the boar organisms with Toxicant-induced reproductive dysfunctions

Abstract

The pathogenetic mechanism of reproductive diseases is oxidative stress, which is manifested by an increase in lipid peroxidation and a decrease in antioxidant potential. The aim of the study was to develop a method for the correction of biochemical changes in the body of boars with toxicant-induced reproductive dysfunctions using drugs based on nanobiomaterials, based on reducing lipoperoxidation, neutralization of toxic substances by antioxidant protection increasing of animals and endocrine activity stimulating of their gonads. The article presents the results of research on the effectiveness of the complex drug ‘Karafand+OV,Zn’, which contains carotenoids, phytoandrogens and nanomaterials — nanoparticles of gadolinium orthovanadate, activated by europium, and zinc carbonate. Experimental toxicant-induced reproductive dysfunctions were caused by feeding sodium nitrate at a dose of 0.3 g NO3–/kg body weight. The drug was administered in a dose of 15 ml per male, orally, once a day for 14 days. Blood samples for test were taken before and on the 20th day after drug administration. Conventional biochemical methods were used, as well as chemiluminometry and enzyme-linked immunosorbent assay. There was a positive effect of the developed drug on the hormonal state (testosterone concentration increased by 91.8% (20.6 ± 0.32 nmol/l, p < 0.001), the content of vitamin A increased 1.3 times (0.65 ± 0.02 μmol/l, p < 0.001) and zinc by 47.6% (24.8 ± 0.86 μmol/l, p < 0.001), the dynamics of lipoperoxidation processes (the concentration of malonic dialdehyde in the serum was reduced by 53.2% (0.394 ± 0.01 μmol/l, p < 0.001)) and the system of antioxidant protection of boars (increased activity of catalase and superoxide dismutase in serum by 71.5% (41.4 ± 1.03 μmol/H2O2/l-min, p < 0.001) and 54.8% (8.98 ± 0.09 st. un./mgHb, p < 0.001), respectively), increased the content of reduced glutathione by 23.2%, indicators of the oxygen metabolism system (concentration 2,3-diphosphoglycerate increased 1.3 times (1.4 ± 0.03 mmol/l, p < 0.001), the activation of which reduces the hypoxic state. The total antioxidant activity of boars increased, as evidenced by a decrease in the light sum of chemiluminescence of serum by 47.6% (4.4 ± 0.15 un., p < 0.001). The results of research convincingly testify to the high efficiency of the use of the complex drug ‘Karafand+OV,Zn’ as a means of correction of toxicant-induced reproductive dysfunctions in boars and proves the possibility of its use in practical veterinary andrology