The effects of vitamins E and C on individual lipides in the liver and skeletal muscles of chicken broilers

Author:

Vishchur O. I.1,Romanovych L. V.2,Smolyaninov K. B.1,Masyuk M. B.1,Romanovych М. М.1

Affiliation:

1. Institute of Animal Biology of the National Academy of Agrarian Sciences of Ukraine

2. Stepan Gzhytskyj National University of Veterinary Medicine and Biotechnologies

Abstract

The purpose of the work was to find out the changes in the lipid composition of the liver and skeletal muscles of broiler chickens at different stages of growth under the influence of supplements of vitamins E and C in their diet. The studies were conducted on four groups of broiler chickens. The control group was fed a standard compound feed. The first experimental group in addition to the specified compound feed received tocopherol acetate in the amount of 0.1 g/kg, the second — ascorbic acid, 0.25 g/kg. The third experimental group of chickens received tocopherol acetate and ascorbic acid at mentioned doses. The lipids from these tissues were extracted by the Folch method, and the ratio of individual lipid classes was determined by thin layer chromatography on silica gel. The results of the studies indicate the stimulating effect of vitamins E and C on the accumulation of total lipids in the skeletal muscles and liver of broiler chickens. In addition, we can conclude that feeding the broiler chickens of supplements containing vitamins E and C during the period of intensive growth promotes the increase of lipid synthesis in their liver and deposition of synthesized lipids in skeletal muscle. However, these processes are accompanied by a redistribution of the ratio of individual lipid classes in the investigated organs and tissues of chickens

Publisher

Kharkiv Entomological Society

Subject

Pharmacology (medical),Complementary and alternative medicine,Pharmaceutical Science

Reference13 articles.

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2. Folch, J., Lees, M. and Sloane Stanley, G. H. (1957) ‘A simple method for the isolation and purification of total lipids from animal tissues’, Journal of Biological Chemistry, 226(1), pp. 497–509. Available at: http://www.jbc.org/content/226/1/497

3. Jiang, Z. G., Robson, S. C. and Yao, Z. (2013) ‘Lipoprotein metabolism in nonalcoholic fatty liver disease’, Journal of Biomedical Research, 27(1), pp. 1–13. doi: https://doi.org/10.7555/JBR.27.20120077

4. Kates, M. (2010) Techniques of Lipidology: Isolation, Analysis, and Identification of Lipids. 3rd ed. Ottawa: NewportSomerville. ISBN 9780981375700

5. Klasing, K. C. (2007) ‘Nutrition and the immune system’, British Poultry Science, 48(5), pp. 525–537. doi: https://doi.org/10.1080/00071660701671336

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