Influence of different doses of feed additive based on silkworm pupae on clinical indicators of rat blood

Abstract

The paper presents data on the effect of different doses of feed additive based on silkworm pupae. A positive correlation between the number of leukocytes and erythrocytes, as well as an increase in the number of agranulocytes compared with granulocytes in the analysis of leukocyte formula, indicate the activation of hematopoiesis, which in turn affects the natural resistance of animals. Under the conditions of the vivarium of the NSC ‘IECVM’ the experimental part of the work on studying the effect of different doses of feed additive based on silkworm pupae was carried out on male Wistar rats (n = 49) aged four months, weighing 175–190 g. Three groups of rats were formed following the principle of analogs. Rats of the control group received a standard diet with free access to water, rats of group I were fed a standard diet with the addition of the feed additive at a dose of 8.0 ml/kg body weight, group II — 12 ml/kg body weight. On days 21 and 41 of the experiment, blood samples were taken from rats during light chloroform anesthesia. The level of hemoglobin in the blood was determined using reagent kits manufactured by PJSC ‘Reagent’ (Ukraine), the total number of leukocytes and erythrocytes was performed according to the generally accepted methods of counting in Goryaev’s chamber. The calculation of the leukocyte formula of the blood of rats was performed on blood smears. Smears were stained by Romanowsky–Giemsa method. Based on the obtained data, integrated hematological parameters and color index were calculated following the methodological recommendations. There was a positive correlation between the number of leukocytes by 13.4% (p ≤ 0.05) and erythrocytes by 39.2% (p ≤ 0.05), as well as an increase in the number of agranulocytes to 50.0% (p ≤ 0.05) than granulocytes in the analysis of leukocyte formula — this indicates the activation of hematopoiesis, which in turn affects the natural resistance of animals. In addition, the index of immunoreactivity according to Ivanov et al. (2002) in the group II of animals had an increase of 61.4% (p ≤ 0.05) relative to control. The obtained results allow us to expand the current knowledge about the effect of the drug based on silkworm pupae on the body of animals, in particular on the clinical parameters of the blood. A positive correlation between the number of leukocytes and erythrocytes, as well as an increase in the number of agranulocytes than granulocytes in the analysis of the leukocyte formula, indicates the activation of hematopoiesis, which in turn affects the natural resistance of animals