Abstract
Physicochemical properties of gliadin in different solvents (dimethyl sulfoxide (DMSO), H2O, and aqueous ethanol) and pH (9.8, 6.8, and 1.2) were investigated using dynamic light scattering (DLS), zeta potential (ZP), and attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR). Gliadin-DMSO and gliadin-deionized water (H2O) (pH 9.8) showed a lower size distribution, whereas samples solubilized in 60% aqueous ethanol presented a lower size distribution only at pH 1.2. ZP analysis showed that gliadin-H2O (pH 9.8) was the most stable evaluated system. ZP results of gliadin-DMSO indicated an unstable system, with the coexistence of several protein conformations. ATR-FTIR analysis showed that, in H2O, most protein conformations were β-sheets, while in DMSO a band at 1660 cm−1 appeared to be related to protein unfolding. The techniques proved to be effective in monitoring conformation and stability of all gliadin/solvent systems. Such information can be used in the development of new gliadin-based materials.
Publisher
Sociedade Brasileira de Quimica (SBQ)
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献