Development and Validation of Analytical Method by HPLC-PDA and Seasonality from Gallic Acid, Catechin, and Epicatechin in Leaf Extracts from Campomanesia adamantium

Author:

Cabral Raphael,Borges Leonardo,de Oliveira Matheus,Romano Camila,Fiuza Tatiana,de Paula JoséORCID

Abstract

Campomanesia adamantium is native of the Brazilian Cerrado, and its leaves are popularly used as anti-inflammatory, antidiarrheal, and antiseptic in the urinary tract. This study aimed to develop and validate an analytical method by high-performance liquid chromatography for the simultaneous quantification and evaluation of the seasonal variability of gallic acid, catechin, and epicatechin, during 12 months, in C. adamantium leaf extracts. Chromatographic separations were performed with a mobile phase gradient of acetonitrile and 0.05% (v/v) trifluoroacetic acid solution, flow rate of 1.0 mL min-1, detection at 210 nm, C18 column (150 × 4.6 mm, 5 μm) and column oven temperature of 35 °C. The analytical method developed was selective, linear, precise, accurate, robust, and without matrix effect. The parameters obtained in the present study meet the requirements established by national and international guidelines. The best time for leaves harvesting, with the highest levels reached, was in September (0.0626%, m/v) and August (0.044%, m/v) for gallic acid, in June (0.3953%, m/v) and July (0.3804%, m/v) for catechin, and from May (0.1622%, m/v) and June (0.1415%, m/v) for epicatechin, in the dry season. Therefore, this study contributes by providing parameters for quality control of the raw material C. adamantium.

Publisher

Sociedade Brasileira de Quimica (SBQ)

Subject

General Chemistry

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3